Screen Drug Test Saliva 6

Rapid screening test for the simultaneous, qualitative determination of amphetamine, methamphetamine, cocaine , opiates, marijuana, ecstasy and their metabolites in human saliva.

Precautions
• Product not intended for medical or diagnostic use.
• Do not use beyond the expiry date.
• Store the test strip panel in the closed pouch until ready to use.
• Store in the sealed pouch until ready to use.
• Saliva is not classified as a biohazardous substance if not derived from a dental procedure.
• The used binder and Card must be disposed of in accordance with the with the regulations in force.


Intended use and summary
Slide chromatographic immunological test for the qualitative identification of multiple drugs and metabolites in saliva at the following threshold-limit concentrations:
< tr>< td> D,L-methylenedioxymethamphetamine

Test< /b>	Calibrator	Threshold-limit (ng/ml)
Amphetamine (AMP)	D-Amphetamine	40
Methamphetamine (MET)	D-Methamphetamine	40
Marijuana (THC)	11-nor-Δ9-THC-9 COOH < /td>	10
Cocaine (COC)	Benzoylecgonine	30
Opiates (OPI/MOP)	Morphine	40
Ecstasy (MDMA)	50

This test provides preliminary analytical data only.
More chemical methods specific methods must be used as confirmation of the analytical result.
Gas chromatography and mass spectrometry (GC/MS) are considered preferential confirmation methods.
Each result for determining the presence of drugs of abuse must be related to clinical considerations and professional judgment, particularly when the preliminary result is of positivity.

Rapid urine screening test that does not require the use of special instruments.
The test uses antibodies to selectively detect high levels of specific substances in human saliva.
Residence times are approximate for each substance as they vary based on frequency of use, body mass, age and age. , state of health, tolerance to narcotic substances.
The product detects the presence of narcotic substances in saliva only after their metabolisation.

COCAINE (COC)
The cocaine is a powerful central nervous system stimulant as well as local anesthetic derived from the coca plant.
The substance is often self-administered via inhalation or intravenous injection or by smoking the base substance.

AMPHETAMINE (AMP
Amphetamine is a substance also used for therapeutic purposes.
The substance it is often self-administered via inhalation or ingestion.
High doses produce enhanced stimulation of the central nervous system and induce euphoria, alertness, reduced appetite, a sense of greater energy and strength.
More acute reactions produce anxiety, paranoia, hallucinations and psychotic behavior.

METHAMPHETAMINE (MET/MDMA)
Methamphetamine is a powerful stimulant chemically related to amphetamine, but with a greater capacity ; stimulation of the central nervous system.
The substance is often self-administered through inhalation, smoking or ingestion.

MARIJUANA (THC)
Tetrahydrocannabinol is the active ingredient of cannabis.
When is it smoked or taken orally THC produces euphoria.
Regular users have short-term memory impairment and learning delay, may also have transient episodes of confusion and anxiety.
Long-term use relatively heavy can be associated with behavioral disorders.

OPIOIDS (OPI)
The class of opiates refers to any substance derived from the opium poppy, including natural compounds such as morphine, codeine and semi-synthetic substances such as heroin.
Opiates act to control pain by depressing the central nervous system.
Withdrawal symptoms may include sweating, tremors, nausea, and irritability.
Opiates can be taken orally or by intravenous, intramuscular and subcutaneous injection.
Consumers can also take the substance intravenously or through inhalation.

ECSTASY (MDMA)
Synthetic drug, first synthesized in 1914 by a’ German pharmaceutical company for the treatment of obesity.
Those who use it regularly report side effects such as increased muscle tension and sweating.
MDMA is not a real stimulant, even if it has in common with amphetamines a capacity to to increase blood pressure and heart rate.
MDMA produces some perceptual changes such as increased sensitivity to in the light, difficulty of concentration and blurred vision in some users.
Its mechanism of action is thought to occur through the release of the neurotransmitter serotonin.
MDMA can also release dopamine, even if it is common opinion that this is a secondary effect of the substance (Nichols and Oberlender, 1990).
The greatest effect manifesto of MDMA, which potentially occurs in all those who have taken a reasonable dose of the substance, is the contraction of the jaws.
The test for MDMA contained in the product provides a positive result when the concentration of MDMA in the saliva exceeds 50 ng/ml.

PRINCIPLE
&` an immunological test based on the principle of competitive binding.
Drugs that may be present in the oral fluid sample compete against their respective conjugates for binding sites on the specific antibody.
During the test, a portion of the saliva sample migrates upward by capillary action.
A drug, if present in the oral fluid sample below the threshold concentration, will not saturate the substance. the binding sites of this specific antibody.
The antibody will therefore react with the drug-protein conjugate and will appear a colored line visible in the test region for the specific drug tested. The presence of the drug above the threshold concentration will saturate the substance. all the binding sites of the antibody.
Therefore the colored line will not form in the test area.
A positive oral fluid sample will not generate a colored line in the specific test area of the strip due to drug competition, while a drug-negative oral fluid sample will generate a a line in the test area for the absence of competition.
For procedural control purposes, a line will appear always a colored line in the control area, indicating that it is a correct volume of sample has been poured and that the membrane is been soaked.

REAGENTS
The test contains membrane strips coated with drug-protein conjugates (purified bovine albumin) on the test line, a goat polyclonal antibody against gold conjugate -protein on the control line, and a swab containing colloidal gold particles coated with mouse monoclonal antibody specific for Amphetamine, Methamphetamine, Cocaine, Opiates, Δ9-thc-cooh, Methadone, Ecstasy and Synthetic Marijuana.

STORAGE AND STABILITY
Store in the original sealed packaging at 2-30°C.
The test is stable until the expiry date printed on the package.
The test must remain in the sealed package until used.
Do not freeze.
Do not use beyond the expiry date.

COLLECTION AND PREPARATION OF SPECIMENS
The oral fluid sample should be collected using the collector provided with the kit.
Follow the detailed instructions below.
Do not use other collection devices for analysis.
Oral fluid may ; be collected at any time of the day.

INSTRUCTIONS FOR USE
Bring the test, sample and/or controls to room temperature (15-30°C) before of the test.
Inform the donor not to put anything in the mouth, including food, drinks, gum or tobacco, for at least 10 minutes before collection.
1. Bring the package to room temperature before opening. Remove the test from the sealed package and use it within one hour.
2. Remove the test cap and insert the absorbent pad into the mouth, under the tongue, to collect saliva until the control line appears. Remove the test.
3. Place the test on a clean, flat surface. See illustration below. 4. Read the results after 10 minutes. Do not read results after 1 hour.

INTERPRETATION OF RESULTS
Negative*: All test bands appear. One colored line must be in the control zone (C), while the other must be adjacent to the test zone (Substance/T). The negative result indicates that the concentration of the substance is lower than the detectable level or absent.
*the shade is in the test area (Substance/T) varies, but must be considered negative even if a faintly colored line appears
Positive: only one colored line appears in the control area (C). No test band (Substance/T) appears in the test region. The positive result indicates that the concentration of the substance is above the detectable level.
Invalid: No control line appears. The most important reasons Insufficient sample volume or incorrect procedural techniques are likely to cause the control line to fail to appear. Check the procedure and repeat the test using a new card.
If the test is positive, avoid aggressive behavior and calmly seek dialogue with the person concerned. Get advice from your doctor, a psychologist or contact the competent service for your area.

QUALITY CONTROL
In the test it is including a procedural check.
The line that appears in the control area (C) is considered an internal procedural control.
Confirms that it is a sufficient volume of sample, adequate imbibition of the membrane and correct procedural technique were used.

LIMITATIONS
1. The test provides only a preliminary qualitative analytical result. &` It is necessary to use a secondary analytical method to confirm the result. The recommended confirmation method is gas chromatography/mass spectrometry (GC/MS).
2. A positive test result does not indicate the concentration of the substance in the sample or the route of administration.
3. A negative result may not necessarily indicate a sample drug-free. &` It is possible to get negative results when the drug is present below the test limit threshold.
4. It is It is possible that technical or procedural errors, as well as such as interfering substances present in the saliva sample, can cause erroneous results.
5. This test does not distinguish between drugs of abuse and some medications.

FORMAT
&bull ; 1 swab.
• 1 Drug Test Saliva 6.
• information leaflet.